Intermediate
Part:BBa_K273014:Design
Designed by: Karl Brune Group: iGEM09_Uppsala-Sweden (2009-10-17)
RBS - pdc - RBS - adh2(Y) - Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1033
Illegal AgeI site found at 1180
Illegal AgeI site found at 1558 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 67
Illegal SapI site found at 481
Illegal SapI site found at 1144
Design Notes
We had to use the adh2 from S. cerevisae as the adh2 from our particular Z. mobilis contained a unexpected EcoRI restriction site.
Source
Assembled by the Uppsala iGEM Team 2009