Intermediate

Part:BBa_K273014:Design

Designed by: Karl Brune   Group: iGEM09_Uppsala-Sweden   (2009-10-17)


RBS - pdc - RBS - adh2(Y) - Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1033
    Illegal AgeI site found at 1180
    Illegal AgeI site found at 1558
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 67
    Illegal SapI site found at 481
    Illegal SapI site found at 1144


Design Notes

We had to use the adh2 from S. cerevisae as the adh2 from our particular Z. mobilis contained a unexpected EcoRI restriction site.


Source

Assembled by the Uppsala iGEM Team 2009

References